Applications for Peridinin Chlorophyll Protein Complex
Peridinin Chlorophyll (PerCP) is commonly used in immunoassays such as FACS and flow cytometry.
Peridinin is a light-harvesting pigment associated with chlorophyll and found in the peridinin chlorophyll binding protein (PerCP); a light harvesting protein found in dinoflagellates. The PerCP complex has a high ratio of peridinin to chlorophyll; most light-harvesting complexes contain more chlorophyll than carotenoids, but the PerCP contains eight peridinin and two chlorophyll molecules arranged to promote peridinin-chlorophyll energy transfer. The PerCP complex is a trimer with a molecular weight of 35kDa.
|Absorption maximum||483 nm||A483/A280 ≥ 4.6|
|Emission maximum||676 nm||Absorbance max. 483 ± 1 nm|
|Extinction Coefficient (ε)||1.96 x 106 M-1cm-1||Emission max. 676 ± 1 nm|
|>95% single peak by HPLC|