Applications for B-Phycoerythrin
Because of its high quantum yield, B-PE is considered the worldâ€™s brightest fluorophore. It is compatible with commonly available lasers and gives exceptional results in flow cytometry, Luminex® and immunofluorescent staining. B-PE is also less â€œstickyâ€? than common synthetic fluorophores and therefore gives less background interference.
B-phycoerythrin (B-PE) is produced by certain red algae such as Rhodella sp. The specific spectral characteristics are a result of the composition of its subunits. B-PE is composed of at least three subunits and sometimes more. The chromophore distribution is as follows: α subunit with 2 phycoerythrobilins (PEB), β subunit with 3 PEB, and the γ subunit with 2 PEB and 2 phycourobilins (PUB). The quaternary structure is reported as (αβ)6γ. The molecular weight of B-PE is approximately 240,000 Daltons.
|Absorption maximum||545 nm|
|Additional Absorption peak||563.5 nm|
|Emission maximum||572 nm|
|Extinction Coefficient (ε)||2.41 x 106 M-1cm-1|
|Quantum Yield (QY)||0.98|
|Brightness (ε x QY)||2.36 x 106 M-1cm-1|
|A545/A280 ≥ 5.5|
|A620/A546 ≤ 0.01|