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SureLight® PE-695

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RPE

Applications for SureLight® PE-695

Many applications and instruments were developed specifically for PE-695. It is commonly used in immunoassays such as FACS, flow cytometry, multimer/tetramer applications. With new instrumetation available, PE-695 is also well suited for immunohistochemistry (IHC) and Western blot applications.

Structural Characteristics

R-phycoerythrin is predominantly produced by red algae. The protein is made up of at least three different subunits and varies according to the species of algae that produces it. The subunit structure of the most common R-PE is (αβ)6γ. The α subunit has two phycoerythrobilins (PEB), the β subunit has 2 or 3 PEBs and one phycourobilin (PUB), while the different gamma subunits are reported to have 3 PEB and 2 PUB (γ1) or 1 or 2 PEB and 1 PUB (γ2). The molecular weight of R-PE is 250,000 Daltons. PE-695 is a tandem fluorophore comprising R-Phycoerythin and a red-shifted cyanine dye.

Spectral Characteristics

Absorption maximum 565 nm
Additional Absorption peak 498 nm
Emission maximum 695 nm

Specifications

Download Info Sheet

excitation emission

Excitation and emission profiles for SureLight® PE-695 (Emission scan excitation wavelength at 488 nm.)

 

 


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