Applications for R-Phycoerythrin
Many applications and instruments were developed specifically for R-phycoerythrin. It is commonly used in immunoassays such as FACS, flow cytometry, multimer/tetramer applications. With new instrumetation available, R-PE is also well suited for immunohistochemistry (IHC) and Western blot applications.
R-phycoerythrin is predominantly produced by red algae. The protein is made up of at least three different subunits and varies according to the species of algae that produces it. The subunit structure of the most common R-PE is (αβ)6γ. The α subunit has two phycoerythrobilins (PEB), the β subunit has 2 or 3 PEBs and one phycourobilin (PUB), while the different gamma subunits are reported to have 3 PEB and 2 PUB (γ1) or 1 or 2 PEB and 1 PUB (γ2). The molecular weight of R-PE is 250,000 Daltons.
|Absorption maximum||565 nm|
|Additional Absorption peak||498 nm|
|Emission maximum||573 nm|
|Extinction Coefficient (ε)||1.96 x 106 M-1cm-1|
|Quantum Yield (QY)||0.84|
|Brightness (ε x QY)||1.65 x 106 M-1cm-1|
|A566/A280 ≥ 5.0|
|A566/A498 < 1.5|
|A620/A566 < 0.01|
|Functional activity Relative QY > 2.50|
|>95% single peak by HPLC|